Abstract |
The effect of fermentation temperature on the production of high content alcohol has been investigated with high substrate concentration. The maximum specific growth rate, umax was 0.461hr^-1 at 35℃ which was the highest, whereas the maximum biomass concentration was 8.7g/l at 25℃, at the growth rate lower than at 35℃. Approximately 140g/l of ethanol was produced in the temperature range of 20 to 25℃ with nearly complete consumption of the substrate. Extended fermentation time has been required at lower temperatures, however, for the maximum values of biomass concentration and alcohol content, hence higher ethanol productivity, as the temperature was elevated to 40℃. The viability of yeasts was greatly improved by lowering the fermentation temperature down to 25℃ and also extended survival of the cells has been observed at lower fermentation temperatures, although the ethanol concentration of both was higher. |