Title 인삼적부병원균의 영양생리학적 연구
Author 유병서 · 이민웅 · 이지열 * · 최혜정
Address 동아대학교 대학원 응용생물학과; 서울여자대학 교양학과 *
Bibliography Korean Journal of Microbiology, 20(3),119-124, 1982
DOI
Key Words The nutritional physiology of red rotting of ginseng cause by erwinia carotovora
Abstract As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the growth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, KH₂PO₄1.0g, sucrose 30.0g, MgSo₄· 7H₂O 0.5g, FeCl₃· 6H₂O 1.0mg thiamine 200γg, and distilled water 1000ml, pH6.5.
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