Title Clostridium botulinum type B 독소의 정제방법에 관한 연구
Author 박문국 · 양규환
Address 한국과학기술원 생물공학과
Bibliography Korean Journal of Microbiology, 20(4),183-189, 1982
Key Words Improved procedure for purification of clostridium botulinum type B toxin
Abstract The neurotoxin of Clostridium botulinum type B was purified from a liquid culture. The purification steps consist of ammonium sulfate precipitation of whole culture, treatment of Polymin P(0.15%, v/v), gel filtration on Sephadex G-100 at pH5.6 and DEAE-Sephadex charomatography at pH8.0. The procedure recovered 17% of the toxin assayed in the starting culture. The toxin was homogeneous by sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis and had a molecular weight of 163,000. Subunits of 106,000 and 56,000 molecular weight were found when purified toxin was treated with a disulfide-reducing agent and electro phoresed on SDS-polyacrylamide gels.
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