Title 한강에서 분리한 이종 coliphage의 동정과 점토질에 대한 흡착 및 용출효과
Author 홍순우 · 하영칠 · 안태석 · 이영숙
Address 서울대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 20(4),210-222, 1982
Key Words Identification of two coliphages from Han-river and its adsorption-elution effect on soil materials
Abstract Coliphages isolated from Han-River from September 1980 to August 1981 were classified by morphological and physiological characteristics. Effects of soil material on the fate of coliphage in nature were investigated. 1. The correlation coefficient between coliphage and E.coli which was host of coliphages in nature was 0.7173 (p=0.004). 2. Coliphage I isolated from Han-River of which DNA molecular weight was 27 × 10^6 daltons was identified as T₁ phage and coliphage II of which DNA molecular weight 72 × 10^6 daltons was classified as T_5 phage. 3. Soil material SW was composed of 63.65% silt and 21.92% clay. Clay was consisted of illite, kaolinite and chlorite evenly. Soil material J was composed of 68.92% silt and 11.67% clay. Clay consisted of smectite only. 4. Coliphage was absorbed to soil material J more than soil material SW, and T₁coliphage was absorbed to soil material more than T_5 coliphage was. 5. The phage adsorption efficiency to soil material was enhanced at lower pH : the phage adsorption efficiency at pH 4 was 27 time higher than at pH 7. 6. The concentration of organic compound was inversely related to the phage adsorption efficiency to soil. 8. Adsorption of phage onto soil material, and elution efficiency of elutants was in the order of D.D.W>tap water>river water>seawater. 9. The higher the concentration of organic compound was, the more were adsorbed phages to soil eluted. 10. Coliphages survived longer in sterile soil suspension than in nonsterile soil material suspension.
Download PDF Kor_200407_210-222p.pdf