| Title | Chlorella의 생활사를 통한 -SH화합물 함량의 변화 | ||
| Author | 최호형 · 이영녹 | ||
| Address | 고려대학교 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 19(1),8-13, 1981 | ||
| DOI | |||
| Key Words | The changes in the amounts of SH compounds in chlorella during the synchronous culture | ||
| Abstract | The content of sulfhydryl compounds in Chlorella cells during the life cycle in the synchronous culture is determined spectrophotomatically at 250nm(pH7.0) using p-CMB as SH-reagent. The changes in the content of-SHl compounds and protein in Chlorella cells is measured during the life cycle in connection with cell division and analyzed. 1) The amounts of total ninhydrin reactive substance increased with growth of cells but increased the more at the L₄stage(cytokinesis stage) than at the L₂stage (nuclear division stage). 2) The sulfhydryl content of Chlorella cells increased strikingly at the L₂stage and decreased markedly at the L₄stage. 3) The amounts of values -SH/protein showed a peak at the L₂stage. The increase of the amount of total-SH compounds of cells during the nuclear division period was considered to be caused by the weighty roles of protein-SH groups for the formation of nuclear division apparatus and for the enzyme activity. | ||
| Download PDF | Kor_190102_8-13p.pdf | ||