| Title | Trichoderma koningii의 protoplast 생성에 관하여 | ||
| Author | 조남진 · 이영하 · 홍순우 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 19(4),186-191, 1981 | ||
| DOI | |||
| Key Words | Formation of protoplast from trichoderma koningii | ||
| Abstract | Protoplast production from Trichoderma koningii ATCC 26113 was investigated for the purpose of doing basic and applied researches by protoplast fusion of the cellulolytic filamentous fungus. High yields of protoplast were obtained by using the 18hr. old mycelia treated with the lytic enzyme Driselase of Kyowa Fermentation Co., Japan, in 0.6M MgSO₄or (NH₄)₂SO₄as osmotic stabilizers. The optimum temperature of mycelial digestion was about 28℃ and the number of protoplast increased rapidly after 3hr. digestion. Protoplasts produced at different periods showed distinct morphological heterogeneities in the whole size and the size of vacuole. | ||
| Download PDF | Kor_190405_186-191p.pdf | ||