Title Piricularia oryzae로부터 추출한 cellulase의 몇가지 성질에 대한 연구
Author 전상윤
Address 연세대학교 생물학과
Bibliography Korean Journal of Microbiology, 17(2),58-64, 1979
DOI
Key Words Studies on some properties of cellulase isolated from pirieularia oryzae
Abstract Studies on some properties of cellulase isolated from Piricularia oryzae. Crude cellulases were prepared from dried rice plant powder (Tong-il, Pal-dal) culture of P. oryzae(N-2, C-8, T-2). The best yield of enzyme was obtained from the medium using Tong-il rice plant powder for P. oryzae cav. N-2 and 2%-sucrose concentration in preculture media. Two units of the enzyme were incubated at 60℃ for 1hour with 1.0ml, 0.6% Na-CMC. The optimum temperature for the enzyme activity was at 60℃ and the optimum pH was at pH4.0. When Na-CMC₂was used as substrate the K_m values of crude enzyme were calculated to be 1.05 × 10^-4 mM and V_max was 2.8 mmole/hour. A 10-fold partial purification was achieved by (NH₄)₂SO₄precipitation followed by column chromatography on DEAE Sephadex A-25.
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