Title |
Piricularia oryzae로부터 추출한 cellulase의 몇가지 성질에 대한 연구 |
Author |
전상윤 |
Address |
연세대학교 생물학과 |
Bibliography |
Korean Journal of Microbiology, 17(2),58-64, 1979 |
DOI |
|
Key Words |
Studies on some properties of cellulase isolated from pirieularia oryzae |
Abstract |
Studies on some properties of cellulase isolated from Piricularia oryzae. Crude cellulases were prepared from dried rice plant powder (Tong-il, Pal-dal) culture of P. oryzae(N-2, C-8, T-2). The best yield of enzyme was obtained from the medium using Tong-il rice plant powder for P. oryzae cav. N-2 and 2%-sucrose concentration in preculture media. Two units of the enzyme were incubated at 60℃ for 1hour with 1.0ml, 0.6% Na-CMC. The optimum temperature for the enzyme activity was at 60℃ and the optimum pH was at pH4.0. When Na-CMC₂was used as substrate the K_m values of crude enzyme were calculated to be 1.05 × 10^-4 mM and V_max was 2.8 mmole/hour. A 10-fold partial purification was achieved by (NH₄)₂SO₄precipitation followed by column chromatography on DEAE Sephadex A-25. |
Download PDF |
Kor_170202_58-64p.pdf |