| Abstract |
During the sporulation stage in Saccharomyces cerevisiae J170, the incorporation of D¹⁴C-glucose into starved cells of sporulation stage as well as the vegetative one is appeared higher at pH 6.0. Glucose transport system, in both the vegetative and sporulation stage, is associated with "energy dependent" as the result of repression by such a respiratory inhibitor as 2,4-dinitrophenol. The Km value of glucose uptake in vegetative stage and sporulation stage was 2.1 mM and 2.5 mM respectively, indicating that the glucose is considerably required for vegetative growth. Competition and countertransport of glucose by frutose and galactose are more distinct in vegetative stage, comparing with sporulation stage. The main sugar components of yeast cells consists of ribose, mannose, and α, β-glucose. Amounts of mannose is lower in the sporulation stage than that in the vegetative stage. |
