Title Mycoplasma pneumoniae의 성장과 형태에 미치는 환경요소적 영향
Author 김치경
Address 오하이오 주립대학교 미생물학과
Bibliography Korean Journal of Microbiology, 16(3),131-139, 1978
DOI
Key Words Effects of environmental factors on growth and morphology of mycoplasma pneumoniae
Abstract Mycoplasma pneumoniae was examined for growth characteristics and morphology when cultivated in several media supplemented with a variety of sera and under different atmospheric conditions. Different formula of the medium as well as different sources of lot numbers of the serum in the same medium exhibited varying effects on growth rate and adherence. When the organisms were cultivated in SSR-2 medium in a normal atmospheric environment or under a facultative anaerobic condition provided with carbon dioxide, they developed filamentous cells with heavy growth, whereas mainly round-shaped cells were produced under strict anaerobic conditions of hydrogen and carbon dioxide. Both morphologies of the organism were transformed by switching the incubation environments. An inverted phasecontrast microscopy using modified petri dishes was excellent to observe single cells and useful to follow the development of the cells. Growth, turbidity, and pneumoniae colonies developed on a solid medium and produced clear when overlaid with sheep blood agar.
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