Title 섬유소분해균의 분리 및 그의 생리학적 특성
Author 홍순우 · 하영칠 · 민경희 * · 이영하
Address 서울대학교 자연과학대학 생물학과; * 숙명여자대학교 문리과대학 생물학과
Bibliography Korean Journal of Microbiology, 14(1),17-24, 1976
Key Words Isolation of cellulolytic microorganisms and their physiological characteristics
Abstract Celluloytic microorgnasims were isolated form the various sources and four of them were identified as Trichoderma koningi, Aspergillus niger, Penicillium chrysogenum, and Streptomyces sp. The induction of extracellular5 cellulase of these species in the liquid culture media containing carboxymethylcellulose (CMC) or Avicel as inducer showed that CMC was a better effective inducer for the production of CMCase(Cx cellulase component) as well as Avicelase(C₁cellulase component) than Avicel. It is believed that certain hydrolysis products of cellulose(CMC) could serve as an inducer for an enzyme synthesis. In T. roningi, Asp. niger, and Strptomyces sp., the optimum temperature of CNCase on CMC-culture medium was 50℃ but temperature around 40℃ was found to be optimum for the activities of CMCase prepared from P.ehrysogenum. The optimum temperature for Avicelase activitiles on Avicel-culture media of T. roningi and P. chrysogenum was 40℃ whereas temperature 50℃ was found to be optimum for Avicelase from A.niger and Streptomyces sp. The optimal activities of these CNCase and Avicelase prepared from. T. ronigi, Pen.chrysogenum and Streptomyces sp. were found similarly to be at pH's around 5.4 and 6.0 while pH 4.8 was optimum for the activities of CMCase and Avicelase from A.niger, indicating that A.niger in acidic media would yield an enzyme of high activity.
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