Abstract |
A number of antibiotics and organic compounds were tested for their effect on the multiplication of tobacco mosaic virus(TMV) in floating leaf discs. 1) Among six antibiotics tested, kanamycin sulfate showed 24% or more inhibition to TMV multiplication under these experimental conditions. Aureomycin hydrochloride and chloramphenicol showed 19% and 14% inhibition respectively. 2) For screening of organic compounds two environments were used, a diffuse day- light environment (25 foot-candles and laboratory temperature) and an artificial light environment for 12 hours per day(300 foot-candles at 4 to 5℃ above laboratory temperature). A wide range of organic compounds increased virus multiplication in the diffuse daylight environment but had less effect, or inhibited virus multiplication, in the artificial light environment. 3) The following compounds were among the most effective in increasing TMV multiplication: glucose-1-phosphate, L-aspartic acid, glucose, and 5-bromouracil. The following compounds were most effective in inhibiting TMT multiplication: thiouracil, uracil, DL-isoleucine, L-leucine, and zinc chloride. |