Title Aspergillus niger group의 dextrinogenic amylase에 관한 연구
Author 김상재 · 이배함 · 이용욱 *
Address 건국대학교 응용미생물연구소; * 서울대학교 보건대학원
Bibliography Korean Journal of Microbiology, 9(4),155-162, 1971
DOI
Key Words A study on dextrinogenic amylase in the aspergillus niger group
Abstract A comparison of dextrinogenic amylase activities in the Asp. niger group was made with their crude and ethanol dialized enzymes before and after heating at high temperature (60-75℃). The results obtained are as follows ; 1. THe dextrinogenic amylase activity of crude enzymes of Asp. kawachii and Asp. foetidus was strong, but Asp. phoernicis, Asp. carbonarius and Asp.japonicus showed weak activity. The others showed medial grades of activity. 2. The ethanol dialized enzymes of Asp. kawachii, Asp. foetidus and Asp. japonicus was very sesitive to high temperature (60 or 65℃) and their enzymatic activities were diminished greatly. The others did not show diminution of enzymatic activity at 60 or 65℃, but diminished greatly at 70 or 75℃. 3. The ethanol dialized enzymes of the Asp.niger group heated to 65℃ was more sensitive at pH 6.0 and 6.5 than at pH 4.5, 5.0 and 5.5. 4. Tested strains in the Asp.niger group were subdivided into 4 subgroups by their dextrinogenic amylase activities before and after heating at 60 or 65℃. The first group showed a medial grade of activity before heating and no diminution of their enzymatic activities after heating. Asp. niger, Asp.pulverulentus, Asp. awamori and Asp. usmii were included in this group. The second group had strong enzymatic activity before heating, but diminished greatly after heating. Asp rawachii and Asp. phoenicis were included in this group. The fourth group showed very weak enzymatic activity before heating, and was inactivated easily by heating. Asp.oryzae of the Asp. flavus group showed a very strong dextrinogenic amylase activity before heating. After the heat treatment, however, its enzymatic activity was diminished greatly.
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