| Title | 제한효소 처리된 Genomic DNA에 의한 Polymerase Chain Reaction 증폭 효율에 관한 연구 | ||
| Author | 민해기 1 * 장영효 2 | ||
| Address | 1Department of Internal Medicine, Virginia Commmonwealth University, PO BOX 980263, Richmond, Virginia 23298 ;2 한국생명공학연구원 생물자원센터 | ||
| Bibliography | Korean Journal of Microbiology, 40(3),254-256, 2004 | ||
| DOI | |||
| Key Words | . | ||
| Abstract | Polymerase chain reaction (PCR) is a powerful tool for precisely amplifying selected DNA sequences that have had a broad impact on genomic studies. When examining human [alpha]- and [beta]- tryptase genes which have 95% DNA homology, inconsistent PCR amplification of genomic sequences hampered our progress. This study suggests that long PCR technique on the original DNA digested with restriction enzymes improves both efficiency and sensitivity of PCR. These improved results seem to derived from the effective denaturation of the original genomic DNA template or reduction of formation of secondary structures that block either primer annealing or extension in PCR. Elimination of homo- or hetero-duplex products derived from highly homologous genes provides an additional advantage in this study. This communication describes how the use of restriction enzymes improved these efficiencies, and also facilitated studies of highly homologous genes including tryptase genes. | ||
| Download PDF | 40(3)-7(254-256)6.pdf | ||