| Title | Genotypic Investigation of Multidrug-Resistant Pseudomonas aeruginosa from Clinical Isolates in Korea, 2010 | ||
| Author | Min Ji Kim1, Min Kyeong Cha1, Do Kyung Lee1, Ju Yeon Kang2, Jae Eun Park1, Young Hee Kim3, Il Ho Park1, Hea Soon Shin2, and Nam Joo Ha1* | ||
| Address | 1College of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea, 2College of Pharmacy, DukSung Women’s University, Seoul 132-714, Republic of Korea, 3College of Nursing, Chung-Ang University, Seoul 156-755, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 48(4),240-245, 2012 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2012.050 | ||
| Key Words | Pseudomonas aeruginosa, antimicrobial resistance, metallo-β-lactamases (MBL), multidrug-resistant | ||
| Abstract | Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes serious infection, particularly in immunocompromised patients. Also, P. aeruginosa possessing carbapenem-resistant metallo-β-lactamases (MBL) has been reported with increasing frequency in Korea. We therefore analyzed the level of multidrug-resistant clinical P. aeruginosa isolated from a secondary hospital in Korea in 2010. A total of 92 isolates of P. aeruginosa were collected from Sahmyook Medical Center in 2010. Susceptibility to antimicrobial agents was determined by analysis of the minimum inhibitory concentration test; the inhibitor-potentiated disk diffusion (IPD) test was performed for MBL detection. RAPD-PCR was used for genotyping to rapidly characterize P. aeruginosa strains isolated from clinical patients. The percentages of non-susceptible isolates were as follows: 40.2% to ceftazidime, 58.7% to meropenem, 56.5% to gentamicin, 46.7% to tobramycin, 62.0% to ciprofloxacin and 97.8% to chloramphenicol. The 29 multidrug-resistant strains were screened by the IPD test: of the 21 PCR-positive isolates, 19 were IPM-1 producers and 2 were VIM-2 producers. Among the 19 IMP-1-producing P. aeruginosa isolates, 16 isolates showed similar patterns, and three different banding patterns were observed. The proportion of IMP-1-producing multidrug-resistant P. aeruginosa from clinical isolates steadily increased in this secondary hospital in Korea in 2010. This study provides information about the antimicrobial-resistant patterns and genotype of multidrug-resistant P. aeruginosa isolated from clinical isolates in Korea, 2010. | ||
| Download PDF | 48(4)_02_p.240-245.pdf | ||