| Title | Note] Construction of Overexpression Vectors and Purification of the Oxygenase Component of Alkylphenol Hydroxylase of Pseudomonas alkylphenolia | ||
| Author | Kyoung Lee | ||
| Address | Department of Microbiology and Biomedical Science Institute at CWNU, Changwon National University | ||
| Bibliography | Korean Journal of Microbiology, 49(1),95-98, 2013 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2013.008 | ||
| Key Words | Pseudomonas alkylphenolia, alkylphenol hydroxylase, bacterial multicomponent monooxygenase (BMM), diiron cluster | ||
| Abstract | Following construction of expression vectors in Escherichia coli, a new procedure involving two-step column purifications with a Fast Performance Liquid Chromatography System was developed for purification of the oxygenase component of alkylphenol hydroxylase of Pseudomonas alkylphenolia. From 50 g wet cake of recombinant E. coli BL21(DE3)(pJJPMO2) cells, 110 mg of pure protein in a heterodimeric form containing a stoichiometric amount of iron were obtained and it exhibited a specific activity of 147 nmole/min/mg. | ||
| Download PDF | 49(1)_15_p.95-98.pdf | ||