| Title | Comparison of Biofilm Formed on Stainless Steel and Copper Pipe Through the Each Process of Water Treatment Plant | ||
| Author | Geun-Su Kim1, Byung-Dae Min2, Su-Jeong Park2, Jung-Hwan Oh3, Ik-Hwan Cho4, Seok-Jea Jang2, Ji-Hae Kim2, Sang-Min Park2, Ju-Hyun Park2, Hyen-Mi Chung2, Tae-Young Ahn5, and Weonhwa Jheong2* | ||
| Address | 1Safety Center, Pulmuone Holdings Co., Ltd., Seoul 120-600, Repulic of Korea, 2Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex, Incheon, Republic of Korea, 3Environmental Analysis & Research team, The Environment Technology Institute, WoongjinCoway CO., Ltd., Seoul 151-919, Republic of Korea, 4Waterworks Head quarters, Incheon, Republic of Korea, 5Department of Microbiology, Dankook University, Cheonan 330-714, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 49(4),313-320, 2013 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2013.3083 | ||
| Key Words | biofilm, copper pipe, DGGE, distribution pipe, drinking water, stainless pipe | ||
| Abstract | Biofilm formed on stainless and copper in water treatment plant was investigated for sixteen weeks. Biofilm reactorwas specially designed for this study. It was similar to that of a real distribution pipe. Raw water, coagulated, settled,filtered and treated water were used in this study. The average number of heterotrophic bacteria counts was 1.6 × 104CFU/ml, 5.8 × 103 CFU/ml, 1.8 × 103 CFU/ml, 1.3 × 102 CFU/ml, 1 CFU/ml, respectively. Density of biofilm bacteriaformed on stainless and copper pipes in raw, coagulated and settled water increased above 2.9 × 103 CFU/cm2 withinsecond weeks while more biofilm bacteria counts were found on the stainless pipe than on the copper pipe. In case offiltered water (free residue chlorine 0.44 mg/L), there was no significant difference in the number of biofilm bacteriaon both pipes and biofilm bacteria below 18 CFU/cm2 were detected on both pipe materials after fifth weeks. Biofilm bacteria were not detected on both pipe materials in treated water (free residue chlorine 0.88 mg/L). According to the results of DGGE analysis, Sphingomonadacae was a dominant species of biofilm bacteria formedon the stainless pipe while the copper pipe had Bradyrhizobiaceae and Sphingomonadaceae as dominant bands. Incase of filtered water, a few bands (similar to Propionibacterium sp., Sphingomonas sp., Escherichia sp., and etc.)that have 16S rRNA sequences were detected in biofilm bacteria formed on both pipes after fifth weeks. Stainlesspipe had higher species richness and diversity than the copper pipe. | ||
| Download PDF | 49(4)_03_p.313-320 with supplementary data.pdf | ||