Title Iron Containing Superoxide Dismutase of Streptomyces subrutilus P5 Increases Bacterial Heavy Metal Resistance by Sequestration
Author Jae-heon Kim*, Kwang yong Han, Ho jin Jung, and Jungnam Lee#
Address Department of Microbiology, Dankook University, Cheonan 330-714, Republic of Korea, #Present address: Department of Periodontology and Oral Biology, University of Florida, Gainesville, Florida, USA
Bibliography Korean Journal of Microbiology, 50(3),179-184, 2014
DOI http://dx.doi.org/10.7845/kjm.2014.4053
Key Words Escherichia coli, Streptomyces subrutilus, heavy metals, resistance, sequestration, superoxide dismutase
Abstract Mitigation of heavy metal toxicity by iron containing superoxide dismutase (FeSOD) of Streptomyces subrutilus P5 was investigated. For E. coli DH5α, the survival rate in the presence of 0.1 mM lead ions was only 7% after 120 min; however, with the addition of 0.1 μM of purified native FeSOD the survival rate increased to 39%. This detoxification effect was also shown with 0.01 mM copper ions (survival increased from 6% to 50%), and the effect was stronger than with the use of EDTA. E. coli M15[pREP4] producing 6xHis-tagged FeSOD was constructed, and this showed an increase in survival rates throughout the incubation time; in the presence of 0.1 mM lead ions,the final increase at 60 min was from 3% to 19%. The FeSOD absorbed about 123 g-atom lead per subunit; therefore, we suggest that FeSOD could sequestrate toxic heavy metals to enhance bacterial survival against heavy metal contamination.
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