Title Characterization of a Small Cryptic Plasmid from Pseudomonas nitroreducens Strain TX1
Author Nguyen Ngoc Tuan1, Kyoung Lee2, Ju Beom Kang2, and Shir-Ly Huang1,3*
Address 1Department of Life Sciences, National Central University, No. 300 Chung-da Rd., Chung-li, 32001, Taiwan, 2Department of Microbiology and Biomedical Institute at CWNU, Changwon National University, Kyongnam 641-773, Republic of Korea, 3Center for Biotechnology and Biomedical Engineering, National Central University, No. 300 Chung-da Rd., Chung-li, 32001, Taiwan
Bibliography Korean Journal of Microbiology, 50(3),210-215, 2014
DOI http://dx.doi.org/10.7845/kjm.2014.4038
Key Words Pseudomonas nitroreducens, cryptic plasmid, rolling circle replication
Abstract Pseudomonas nitroreducens TX1 was isolated from a rice field drainage in Taiwan. The bacterium is of special interest because of its capability to use a group of nonionic surfactants such as alkylphenol polyethoxylates even at high concentrations as a sole carbon source. In this study, a small cryptic circular plasmid, pTX1, was characterized from P. nitroreducens TX1. It is 2,286 bp in length with a GC content of 63.3% and harbors three open reading frames, ReppTX1 and functionally unidentified ORF1 and ORF2. The predicted reppTX1 gene product is homologous to Rep proteins of plasmids belonging to the pC194/pUB110 family, which is predominantly found in Gram-positive bacteria and is known to replicate by the rolling-circle mechanism. The copy number of pTX1 was estimated to be about 150 in each cell. Based on the genetic fingerprints and comparison with other plasmids, it is concluded that pTX1 replicates by a rolling circle mechanism which is rarely found for Pseudomonas plasmids.
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