| Title | Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat | ||
| Author | Jae Eun Park1, Do Kyung Lee1, Min Ji Kim1, Kyung Tae Kim2, Kyung Soon Choi3, Jae Goo Seo4, and Nam Joo Ha1* | ||
| Address | 1College of Pharmacy, Sahmyook University, Seoul 139-742, Republic of Korea, 2Department of Chemistry, Sahmyook University, Seoul 139-742, Republic of Korea, 3Department of Food and Nutrition, Sahmyook University, Seoul 139-742, Republic of Korea, 4R&D Center, Cellbiotech, Co. Ltd., Gimpo, Gyeonggi 157-030, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 50(4),296-301, 2014 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2014.4064 | ||
| Key Words | anti-rotaviral activities, probiotics, real-time quantitative PCR (RT-qPCR) | ||
| Abstract | Rotavirus is a major cause of acute gastroenteritis in young children in developed and developing countries. The use of probiotics for the treatment of gastrointestinal diseases is both safe and easily accessible. In this study, we evaluated the anti-rotaviral activities of probiotic mixtures in a Sprague-Dawley rat. 24 litters with their dams were randomly assigned to four groups; placebo, phosphate buffered saline (PBS), and two probiotic mixture (PRO-1 and PRO-2) groups. All rats were inoculated with rotavirus at dose of 8 log plaque forming units per rat at 5 days old. Animals in the PRO-1 and PRO-2 groups were orally administered probiotic mixtures 1 or 2, respectively, at a dose of 8 log colony forming units daily during 4 days. For control purposes, placebo and PBS groups were orally administered the same amount of placebo (containing maltose and polydextrose) or PBS once daily for 4 days, respectively. Antiviral analysis was performed by real-time quantitative PCR (RT-qPCR) and observing intestinal villi. As a result, weights of small intestines were greater in the PRO-1, PRO-2 groups than in control groups. Villi were short and villous epithelial necrosis was exhibited in control groups, but these morphological changes were not observed in PRO-1, PRO-2 treated rats. RT-qPCR analysis showed that VP7 gene level of rotavirus in fecal samples and small intestinal epithelial cells were lower in the PRO-1 and PRO-2 groups. These findings suggest that probiotic mixtures may be useful probiotics for the treatment of or as alternative therapies for rotaviral gastroenteritis. | ||
| Download PDF | 50(4)_05_p.296-301.pdf | ||