| Title | Microbial Diversity in the Enrichment Cultures from the Fermented Beverage of Plant Extract Using Ribosomal RNA Sequence Analysis | ||
| Author | Choung Kyu Lee1, Baolo Kim2, Young Min Kang3, Hee Yul Lee2, Chung Eun Hwang2, Min Ju Ahn2, Weon Taek Seo2, and Kye Man Cho2* | ||
| Address | 1Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, 2Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, 3Basic Herbal Medicine Reserch Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine Medicine (KIOM), Daejeon 305-811, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 50(4),351-359, 2014 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2014.4034 | ||
| Key Words | 16S and 26S rRNA genes, fermented beverage of plant extract, lactic acid bacteria, microbial diversity, yeast | ||
| Abstract | A beverage was produced by the fermentation of mixed extracts from the various fruits, vegetables, algae, and medical herbs. The physicochemical properties of the fermented beverage of plant extracts (FBPE) and microbial diversity were analyzed in cultures enriched from FBPE using 16S and 26S rRNA gene sequence analyses. The pH, acidity, °brix, reducing sugar, and alcohol contents of the FBPE were determined to be the 3.48, 1.68%, 70.0, 1,026 g/L, and 3.5%, respectively. The most abundant free sugar and organic acid in the FBPE were glucose (567.83 g/L) and tartaric acid (93.68 mg/L), respectively. Lactobacillus homohiochii was the predominant species in all enriched culture samples: 100% of the species in 0B (0% sugar) and 40B (40% sugar) libraries and 95.6% of 20B library (20% sugar). Lactobacillus fructivorans was detected in the 20B library. The predominant species in the samples of enrichment cultures collected from FBPE with three different sugar concentrations were: Candida zeylanoides (45.2%) in the 0Y library (0% sugar), Candida lactis-condensi (35.7%) and C. zeylanoides (35.7%) in the 20Y library (20% sugar), and C. lactis-condensi (38.1%) in the 40Y library (40% sugar). This result may provide a useful frame of reference for further analyses of microbial population dynamics in FBPE. | ||
| Download PDF | 50(4)_13_p.351-359.pdf | ||