| Title | Microcystis aeruginosa에 대한 hydrotrope-combined copper의 생장억제 및 독성 평가 | ||
| Author | 박세근1, 지준구1, 장희정2, 김영관3, 오영숙4, and 최성찬1* | ||
| Address | 1한림대학교 환경생명공학과, 2㈜소프트아쿠아, 3강원대학교 환경공학과, 4명지대학교 환경에너지공학과 | ||
| Bibliography | Korean Journal of Microbiology, 51(1),7-13, 2015 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2015.4080 | ||
| Key Words | Microcystis aeruginosa, algicide, Ames test, microcystin, toxicity | ||
| Abstract | Hydrotrope-combined copper (HCC) is a copper (Cu2+)-based algicide, which is combined with a hydrotrope that keeps copper ion in solution to improve performance. This study assessed the growth inhibition effect of HCC against Microcystis aeruginosa which is one of the most common toxic cyanobacterium in eutrophic freshwater environment. Various HCC doses, ranging from 5.5 to 550 μg/L as Cu2+, were applied to either BG-11 or 1/4 diluted medium with low- or high-inoculum density of M. aeruginosa. Growth inhibition was monitored based on a decrease in chlorophyll-a content in culture medium during the incubation. Results showed that HCC significantly inhibited the growth of M. aeruginosa in a dose-dependent manner. In case of 1/4 diluted BG-11 medium, HCC dose as low as 5.5 μg Cu2+/L completely inhibited the production of chlorophyll-a by M. aeruginosa. It was found that HCC did not induce any significant release of microcystin-LR from M. aeruginosa. Acute toxicity of HCC was tested using Daphnia magna, and the 24-h EC50 value was 0.30 mg/L as Cu2+ which was much higher than the actual inhibition dose. Ames test was performed using Salmonella enterica serovar Typhimurium TA100, and HCC showed no increase in the number of revertant colonies. The result suggested that HCC does not have any mutagenic potential in the aquatic environment. In addition, no genotoxic effect of HCC was also confirmed based on the SOS ChromoTest using Escherichia coli PQ37. Therefore, HCC could be used as a relatively safe and effective pre- and post-treatment agent to control hazardous algal blooming in aquatic environments. | ||
| Download PDF | 51(1)_02_p.7-13.pdf | ||