| Title | 동해안 자생식물로부터 분리된 내생균류의 식물생장촉진활성 및 동정 | ||
| Author | 유영현1,2, 진용주3, 강상모4, 오세종2, 이명철2*, and 김종국1* | ||
| Address | 1경북대학교 생명과학부, 2농촌진흥청 국립농업과학원 농업유전자원센터, 3국립농업과학원 농업미생물과, 4경북대학교 응용생명과학부 | ||
| Bibliography | Korean Journal of Microbiology, 51(1),14-20, 2015 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2015.5005 | ||
| Key Words | Penicillium spinulosum, endophytic fungi, gibberellin, plant growth-promoting activity | ||
| Abstract | Coastal plant species, Plantago camtschatica Cham. native to the coastal region of the East Sea were sampled and then morphologically different 20 endophytic fungal strains were purely isolated. Phylogenetic analysis of isolates was done by the Bayesian program based on sequenced internal transcribed spacer (ITS-rDNA) region. Culture filtrates of each of 20 isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity, respectively. As the results, E/PC/10/1 strain showed the highest plant growth-promoting activity among them. The culture filtrate of the strain E/PC/10/1 was revealed as containing gibberellins (GA1, GA3, GA4) by using HPLC, and gas GC/MS with selected ion monitoring (SIM). Finally, this strain was identified as novel Penicillium spinulosum species that producing new GAs with microscopic observation and further molecular analysis with beta-tubulin gene sequence. | ||
| Download PDF | 51(1)_03_p.14-20.pdf | ||