| Abstract |
Since the banning of antibiotic growth promoters (AGPs), the death of livestock has been increased, thus there is a strong
demand for AGP-alternatives. Modulation of gut microbiota has been reported to affect host physiological functions and suggested to be
a novel approach for developing AGP-alternatives. However, little has been understood about livestock gut microbiota compared to that
of humans. We conducted preliminary study provide fundamental information regarding to regional differences in swine gut microbiota.
Swine fecal samples were obtained from farms in Jeju (n=40), Gwangju (n=28), and Haenam (n=30). MiSeq was used to sequence 16S
rRNA V4 region, and Mothur pipeline (Schloss et al., 2009) was used for data processing. A total of 5,642,125 reads were obtained and
3,868,143 reads were remained after removing erroneous reads. Analysis of taxonomic composition at the phylum level indicated greater
abundance of Firmicutes among Jeju swine, and cluster analysis of distribution of operational taxonomic units also showed regional
differences among swine gut microbiota. In addition, correlation analysis between non-metric multidimensional scaling and abundance
of phyla suggested that the phyla Actinobacter, Verrucomicrobia, Firmicutes, and Fibrobacteres were driving factors for the regional
differences.
Livestock gut microbiota may be affected by diet and practices in farms. Our results indicated significant regional differences in swine gut
microbiota, suggesting that future livestock gut microbiota studies should be designed with the regional differences in mind. |
