| Title | 독도 해안식물로부터 분리된 호염성 세균들의 특성 및 계통학적 분석 | ||
| Author | 유영현1,2†, 박종명3†, 이명철3, and 김종국1* | ||
| Address | 1경북대학교 생명과학부, 2농촌진흥청 국립농업과학원 농업유전자원센터, 3롯데중앙연구소 | ||
| Bibliography | Korean Journal of Microbiology, 51(1),86-95, 2015 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2015.5008 | ||
| Key Words | coastal plant, Dokdo islands, halophilic bacteria, phylogenetic analysis | ||
| Abstract | To study the halobacterial diversity at the rhizospheric soil of coastal plant native to Dokdo islands, several host plant were selected and its rhizospheric soil was sampled. Soil sample was diluted serially and pure isolation was done by sub-culture using marine agar media. 26 halophilic strains cultivable at the marine medium containig concentration of 9.0% sodium chloride were selected among total 161 isolates. Their partial 16S rRNA gene sequences extracted from genomic DNA were analyzed and partially identified. Furthermore, to identify their genetic relationship, phylogenetic tree was deduced. Total 26 strains were belongs to Firmicutes (30.8%), Gamma proteobacteria (53.8%), Bacteroidetes (7.7%), Alpha proteobacteria (7.7%), and Actinobacteria (7.7%). These results showed the specific difference from previous researches which has been reported the microbial flora of soil or sea water around the Dokdo islands. Furthermore, 4 among 26 halophilic strains grew at above 12.0% NaCl concentrated marine broth, and 2 strains Idiomarina abyssalis LM4H23 and Halomonas huangheensis AS4H13 grew at 15.0% concentration. These halophilic strains thought to overcoming the severe stress like high salt concentration or variation derived from Dokdo-specific climate and might have unknown, specific relationship with their host coastal plant native to Dokdo islands. | ||
| Download PDF | 51(1)_12_p.86-95.pdf | ||