Title Molecular diversity of endobacterial communities in edible part of King oyster mushroom (Pleurotus eryngii) based on 16S rRNA
Author Choung Kyu Lee1, Md. Azizul Haque2, Byoung Rock Choi3, Hee Yul Lee2, Chung Eun Hwang2, Min Ju Ahn2, and Kye Man Cho2*
Address 1Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, 2Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, 3Department of Future Farming, Sacheon Agricultural Development & Technology Center, Sacheon 664-951, Republic Korea
Bibliography Korean Journal of Microbiology, 51(2),148-155, 2015
DOI http://dx.doi.org/10.7845/kjm.2015.4086
Key Words Pleurotus eryngii, 16S rRNA gene, bacteria diversity, endobacteria, phylogenetic tree
Abstract The diversity of endobacteria in the edible part (cap and stipe) king oyster mushroom (Pleurotus eryngii) was investigated using 16S rRNA sequence analysis. The bacterial 16S rRNA libraries were constructed from the body cap (BC) and the body stipe (BS) of the king oyster mushroom. The twenty sequenced BC clones were divided into four groups and the largest group was affiliated with the Firmicutes (40% of clones). While, the twenty sequenced BS clones could be divided into six groups and the largest group was affiliated with the Actinobacteria (40% of clones). The predominant bacterial family from both the cap and stipe of the mushroom was corresponded with the Gram positive bacteria (62.5%).
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