Title |
Temperature dependent 2,3-dihydroxybenzoic acid production in Acinetobacter sp. B-W |
Author |
Kyoung-Ja Kim1*, Jae-Hun Lee1, and Yong-Joon Yang2 |
Address |
1Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University, Asan 336-745, Republic of Korea, 2Department of Plant and Food Science, Sangmyung University, Cheonan 330-720, Republic of Korea |
Bibliography |
Korean Journal of Microbiology, 51(3),249-255, 2015 |
DOI |
http://dx.doi.org/10.7845/kjm.2015.5033
|
Key Words |
Acinetobacter sp., catechol type, 2,3-dihydroxybenzoic acid, siderophore, temperature dependent |
Abstract |
A soil microorganism producing iron chelator (siderophore) under low iron stress (up to 2 μM of iron) was identified as
Acinetobacter sp. B-W by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron
microscope. Catechol nature of siderophore was detected by Arnow test. Although optimal cell growth was identified at 36°C in
iron-limited media, significant quantities of siderophore were produced only at 28°C. Biosynthesis of siderophore was strongly inhibited
by growth at 36°C. Production of siderophore was completely inhibited by 10 μM FeCl3. Iron chelator produced from Acinetobacter sp.
B-W was purified from supernatant using butanol extraction, Sephadex LH-20 column chromatography and HPLC. Purified sideropore
was identified as 2,3-dihydroxybenzoic acid by HPLC, TLC and IR analysis. |
Download PDF |
51(3)_08_p.249-255.pdf |