Title Succession of bacterial community structure during the early stage of biofilm development in the Antarctic marine environment
Author Yung Mi Lee1,2, Kyung Hee Cho1, Kyuin Hwang1, Eun Hye Kim1, Mincheol Kim3, Soon Gyu Hong1*, and Hong Kum Lee1*
Address 1Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea, 2School of Biological Sciences, College of Natural Science, Seoul National University, Seoul 08826, Republic of Korea, 3Arctic Research Center, Korea Polar Research Institute, Incheon 21990, Republic of Korea
Bibliography Korean Journal of Microbiology, 52(1),49-58, 2016
DOI http://dx.doi.org/10.7845/kjm.2016.6005
Key Words Bacteroidetes, Pseudoalteromonas, Antarctica, biofilm succession, bacterial community, pyrosequencing
Abstract Compared to planktonic bacterial populations, biofilms have distinct bacterial community structures and play important ecological roles in various aquatic environments. Despite their ecological importance in nature, bacterial community structure and its succession during biofilm development in the Antarctic marine environment have not been elucidated. In this study, the succession of bacterial community, particularly during the early stage of biofilm development, in the Antarctic marine environment was investigated by pyrosequencing of the 16S rRNA gene. Overall bacterial distribution in biofilms differed considerably from surrounding seawater. Relative abundance of Gammaproteobacteria and Bacteroidetes which accounted for 78.9–88.3% of bacterial community changed drastically during biofilm succession. Gammaproteobacteria became more abundant with proceeding succession (75.7% on day 4) and decreased to 46.1% on day 7. The relative abundance of Bacteroidetes showed opposite trend to Gammaproteobacteria, decreasing from the early days to the intermediate days and becoming more abundant in the later days. There were striking differences in the composition of major OTUs (≥ 1%) among samples during the early stages of biofilm formation. Gammaproteobacterial species increased until day 4, while members of Bacteroidetes, the most dominant group on day 1, decreased until day 4 and then increased again. Interestingly, Pseudoalteromonas prydzensis was predominant, accounting for up to 67.4% of the biofilm bacterial community and indicating its important roles in the biofilm development.
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