| Title | Effect of plasmid curing on the 2, 3-dihydroxybenzoic acid production and antibiotic resistance of Acinetobacter sp. B-W | ||
| Author | Kyoung-Ja Kim1*, Jin-Woo Kim1, and Yong-Joon Yang2 | ||
| Address | 1Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University, Asan 31538, Republic of Korea, 2Department of Plant and Food Science, Sangmyung University, Cheonan 31068, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 52(3),254-259, 2016 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2016.6043 | ||
| Key Words | Acinetobacter sp. B-W, antibiotic resistance, 2, 3-dihydroxybenzoic acid, plasmid curing | ||
| Abstract | Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at 43°C was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and MnSO4 containing medium at 28°C for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli DH5α was transformed with plasmid isolated from strain B-W. The transformant E. coli DH5α harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli DH5α produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid. | ||
| Download PDF | 52(3)_03_p.254-259.pdf | ||