| Title | Thermoresistant properties of bacterioferritin comigratory protein against high temperature stress in Schizosaccharomyces pombe | ||
| Author | In Wang Ryu1, Su Hee Lee1, Hye-Won Lim2, Kisup Ahn3, Kwanghark Park1, Jae-Hoon Sa4, Kyung Jin Jeong4, Chang-Jin Lim2,5, and Kyunghoon Kim1* | ||
| Address | 1Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea, 2Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea, 3Department of Health Administration, Baekseok Culture University, Cheonan 31065, Republic of Korea, 4Division of Food Analysis, Gangwon Institute of Health & Environment, Chuncheon 24203, Republic of Korea, 5Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 52(4),398-405, 2016 | ||
| DOI | https://doi.org/10.7845/kjm.2016.6062 | ||
| Key Words | Schizosaccharomyces pombe, bacterioferritin comigratory protein, glutathione, reactive oxygen species, thermal stress | ||
| Abstract | The Schizosaccharomyces pombe structural gene encoding bacterioferritin comigratory protein (BCP) was previously cloned using the shuttle vector pRS316 to generate the BCP-overexpressing plasmid pBCP10. The present work aimed to evaluate the thermoresistant properties of BCP against high temperature stress using the plasmid pBCP10. When the S. pombe cells were grown to the early exponential phase and shifted from 30°C to 37°C or 42°C, the S. pombe cells harboring pBCP10 grew significantly more at both 37°C and 42°C than the vector control cells. After 6 h of the shifting to higher incubation temperatures, they contained the lower reactive oxygen species (ROS) and nitrite content, an index of nitric oxide (NO), than the vector control cells. After the temperature shifts, total glutathione (GSH) content and total superoxide dismutase (SOD) activities were much higher in the S. pombe cells harboring pBCP10 than in the corresponding vector control cells. Taken together, the S. pombe BCP plays a thermoresistant role which might be based upon its ability both to down-regulate ROS and NO levels and to up-regulate antioxidant components, such as total GSH and SOD, and subsequently to maintain thermal stability. | ||
| Download PDF | 52(4)_02_398-405.pdf | ||