| Title | Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W | ||
| Author | Kyoung-Ja Kim1*, Ju-Ho Jang1, and Yong-Joon Yang2 | ||
| Address | 1Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University, Asan 31538, Republic of Korea, 2Department of Plant and Food Science, Sangmyung University, Cheonan 31066, Republic of Korea | ||
| Bibliography | Korean Journal of Microbiology, 53(2),97-102, 2017 | ||
| DOI | https://doi.org/10.7845/kjm.2017.7023 | ||
| Key Words | Acinetobacter sp. B-W, L-glutamic acid, siderophore, sole carbon and nitrogen sources | ||
| Abstract | Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at 28°C. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at 28°C was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at 36°C, significant quantities of siderophore were produced only at 28°C. Biosynthesis of siderophore was strongly inhibited by growth at 36°C. Production of siderophore was completely inhibited by 10 μM FeCl3. | ||
| Download PDF | 53(2)_04_p.97-102.pdf | ||