Title |
Identification of Lactobacillus spp. associated with nematodes in peach farm soil |
Author |
Woo-Hyun Lee, Jae Im Choi, Jin Il Lee, Won-Pyo Lee, and Sung-Sik Yoon* |
Address |
Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju 26493, Republic of Korea |
Bibliography |
Korean Journal of Microbiology, 53(3),163-169, 2017 |
DOI |
https://doi.org/10.7845/kjm.2017.7030
|
Key Words |
Lactobacillus plantarum, MALDI-TOF MS, multiplex
PCR, peach farm soil, RAPD-PCR |
Abstract |
Strains D4 and D5 were isolated from peach-rotten soil during
the peach harvest season. The isolates were identified based on
morphological and biochemical characterization, and identification
was determined by 16S rRNA gene sequencing. Results showed
that D4 has high similarity to Lactobacillus plantarum ATCC
14917
T
and Lactobacillus pentosus ATCC 8041
T
at 99.05%
and 98.98%, respectively. D5 was also similar to Lactobacillus
pentosus ATCC 8041
T
and Lactobacillus plantarum ATCC
14917
T
at 98.71% and 98.64%, respectively. In contrast, isolates
showed differences in carbohydrate utilization in comparison
to Lactobacillus plantarum ATCC 14917
T
and Lactobacillus
pentosus ATCC 8041
T
. In view of this we performed VITEK
MS matrix-assisted laser desorption ionization-time of flight
mass spectrometry (MALDI-TOF MS) analysis, multiplex PCR
fingerprinting, and random amplified polymorphic DNA (RAPD)-
PCR to further confirm the identification of D4 and D5. The
results of these analyses showed that both strains were most
similar to Lactobacillus plantarum. |
Download PDF |
53(3)_03_p.163-169[HM2017-030].pdf |