| Title | 포도 과피에서 분리한 효모 Pichia manshurica와 Pichia terricola의 생리활성 증진효과 | ||
| Author | 박상국1, 김동민2, and 오계헌1* | ||
| Address | 1순천향대학교 자연과학대학 생명시스템학과, 2도쿄대학교 생명공학과 | ||
| Bibliography | Korean Journal of Microbiology, 53(4),235-241, 2017 | ||
| DOI | https://doi.org/10.7845/kjm.2017.7058 | ||
| Key Words | Pichia, grape pericarp, physiological activity, yeast | ||
| Abstract | The purpose of this study was to investigate the enhanced physiological activities of two Pichia strains, yeasts isolated from grapes pericarp. Based on phylogenetic analysis using 18S rRNA sequencing, two isolates were identified as Pichia manshurica GU-3 and Pichia terricola GU-4, respectively. The scanning electron microscopic analysis showed that the two isolates grown on YPD medium were of typical elliptical shape with buds and bud scars on cell surface. Physiological activities of the single and mixed Pichia cultures were monitored and compared. In mixed cultures after 72 h of incubation, the maximum activities of tyrosinase inhibition, ACE inhibition, and antioxidant were 81.7%, 45.9%, and 42.7%, respectively. Superoxide dismutase-like activity was approximately 30% in the mixed cultures. These studies demonstrate that Pichia species cultured in the form of mixture can enhance the physiological activities and has potential for the development of new bioactive products. | ||
| Download PDF | 01_[235-241]17-058.pdf | ||