Title 식물 생장을 촉진할 수 있는 구리 내성 세균의 분리
Author 김민주 and 송홍규*
Address 강원대학교 생명과학과
Bibliography Korean Journal of Microbiology, 53(4),251-256, 2017
DOI https://doi.org/10.7845/kjm.2017.7066
Key Words ACC deaminase, antioxidant capacity, Cu-resistant bacteria, plant growth promotion, siderophore
Abstract Some rhizobacteria were isolated, that have copper resistance and can confer copper resistance to plants allowing growth under copper stress. Isolated strains Pseudomonas veronii MS1 and P. migulae MS2 produced 0.13 and 0.26 mmol/ml of siderophore, that is a metal-chelating agent, and also showed 64.6 and 77.9% of biosorption ability for Cu in 20 mg/L Cu solution, respectively. Copper can catalyze a formation of harmful free radicals, which may cause oxidative stress in organisms. Removal activity of 1,1-diphenyl-2-picryl hydrazyl radical and antioxidant capacity of strains MS1 and MS2 increased up to 82.6 and 78.1%, respectively compared to those of control at 24 h of incubation. They exhibited 7.10 and 6.42 μmol α-ketobutyrate mg/h of 1-aminocyclopropane-1-carboxylic acid deaminase activity, respectively, which reduced levels of stress hormone, ethylene in plants, and also produced indole- 3-acetic acid and salicyclic acid that can help plant growth under abiotic stress. All these results indicated that these copper-resistant rhizobacteria could confer copper resistance and growth promotion to plants.
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