| Title | 프로바이오틱 유산균의 바이오제닉 아민 분해능에 영향을 미치는 배양 조건 | ||
| Author | 임은서* | ||
| Address | 동명대학교 식품영양학과 | ||
| Bibliography | Korean Journal of Microbiology, 53(4),273-285, 2017 | ||
| DOI | https://doi.org/10.7845/kjm.2017.7068 | ||
| Key Words | bacteriocin, biogenic amine, lactic acid bacteria, probiotic | ||
| Abstract | The purpose of this study was to investigate the inhibitory effect of antibacterial substances produced by probiotic lactic acid bacteria (LAB) against biogenic amines-producing bacteria and the influence of culture conditions on the antibacterial activity of bacteriocin and organic acid. The bacteriocin solutions of Lactobacillus plantarum FIL20 (64 AU/ml) and Lactobacillus paracasei FIL31 (128 AU/ml) showed strong antibacterial activity against Serratia marcescens CIH09 and Aeromonas hydrophilia RIH28, respectively. And the lactic acid contents in the cell-free culture supernatants (CFCS) obtained from FIL20 and FIL31 strains were 107.3 ± 2.7 mM and 129.5 ± 4.6 mM, respectively. Therefore, the bacteriocin solution (200 AU/ml) and the CFCS (200 μl/ml) produced by L. plantarum FIL20 and L. paracasei FIL31 significantly (P < 0.05) decreased the bacterial numbers and histamine and tyramine production ability of S. marcescens CIH09 and A. hydrophilia RIH28. The amounts of histamine and tyramine produced by the CIH09 strain under conditions of low initial pH (5.0) and incubation temperature (15°C) was significantly reduced by treatment with bacteriocin solution and CFCS obtained from L. plantarum FIL20. In addition, the bacterial counts and biogenic amines contents of CIH09 strain were significantly decreased (P < 0.05) when sodium chloride (5%) or potassium nitrite (200 mg/g) were mixed with the antibacterial substances of L. plantarum FIL20. Consequently, the bacteriocin and organic acid solution of L. plantarum FIL20 and L. paracasei FIL31 can be used as a biological preservation to effectively control the production of biogenic amines by the application of hurdle technology. | ||
| Download PDF | 06_[273-285]17-068.pdf | ||