| Abstract |
In eukaryote, THO/TREX complex plays a critical role in
transcriptional elongation, pre-mRNA processing, and nuclear
mRNA export. This complex is evolutionally well- conserved,
but there are some differences in composition and function
according to organisms. Here we showed that spTex1, a
component of THO/TREX complex, is not essential for growth
and mRNA export in a fission yeast, Schizosaccharomyces
pombe, which is more similar to higher eukaryote than budding
yeast. Deletion and overexpression of the spTex1 gene do not
lead to any detectable growth phenotype and accumulation of
poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is
localized mainly in the nucleus. Yeast two-hybrid and Coimmunoprecipitation
analysis showed that the spTex1 protein
interacted with spHpr1 (THOC1) and spTho2 (THOC2), main
subunits of THO complex. We conclude that the S. pombe Tex1
is a component of THO/TREX complex, but does not plays
important roles in growth and bulk mRNA export from the
nucleus. |
