| Title | Role of Mg^2+ in RNA Splicing of T4 td Intron |
|---|---|
| Author | Sung, Jung Suk · Shin, Sookc¹ · Park, In Kook * |
| Address | Department of Applied Biology, Dongguk University; Department of biology, Sahmyook University |
| Bibliography | Journal of Microbiology, 33(2),160-164, 1995, |
| DOI | |
| Key Words | T4 phage td intron, Mg^2+ ion, RNA splicing |
| Abstract | The splicing activity of T4 phage td intron RNA has been examined with various Mg^2+ ions such as MgCl₂, MgSO₄and magnesium acetate using various splicing conditions such as different incubation time and temperature. The maximum splicing of td intron RNA occurred at the concentration of 5 mM MgCl₂. Raising the Mg^2+ concentration up to 15 mM appeared to promote P2 delection mutant to overcome the loss of some splicing activity. In both wild type and mutant, a complete hydrolysis of RNA occurred at 30 mM MgCI₂MgSO₄and magnesium acetate exhibited the rate and pattern of RNA splicing identical to MgCI₂. The optimal splicing conditions involve the incubation of RNA with 5 mM MgCI₂ at 58℃ for 15 min. The results suggest that Mg^2+ may play a key role in the catalytic mechanism of td intron RNA. |
| Download PDF | Eng_330214_160-164p.pdf |