| Title | Identification of hemolysin as one of the important virulent factors in vibrio anguillarum V7 |
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| Author | Choe, Young Chool · Jeong, Ga Jin * |
| Address | Department of Microbiology; Research Center for Molecular Microbiology, Seoul National University |
| Bibliography | Journal of Microbiology, 33(4),283-288, 1995, |
| DOI | |
| Key Words | Vibrio anguillarum, hemolysin, iron deficiency |
| Abstract | We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23℃ which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R_f) on non-denaturing gel was 0.7. Addition of EDTA and FeCI₃drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animals upon infection. |
| Download PDF | Eng_330404_283-288p.pdf |