| Title | Cloning of the gense coding for extracellular proteases from alkalophilic xanthomonas SP. JK311 |
|---|---|
| Author | Kim, Young Hun · Jang, Ji Yeon · Yeeh, Yeehn¹ · Kim, Yong Ho² · Kim, Sang Hae * |
| Address | Department of Biology; ¹Institute of Basic Science; ²Department of Medical Laboratory Science, Inje Universityy |
| Bibliography | Journal of Microbiology, 33(4),344-349, 1995, |
| DOI | |
| Key Words | Xanthomonas sp. JK311, alkaline proteases, SDS-resistance, protease gene cloning |
| Abstract | The alkalophilic bacterium, Xanthomonas sp. JK311, producing extracellular proteases, was isolated from soil. Xanthomonas sp. JK311 produced five extracellular proteases that are all metalloproteases. Four of them were resistant against 1% SDS. Chromosomal DNA of the Xanthomonas sp. JK311 was digested with BamHI and cloned into PUC19. Among E. coli strain HB101 transformants, a clone secreting the proteases was screened through halo formation on skim-milk agar plate and by Southern blot analysis. It had the recombinant plasmid pXEP-1 containing the 7.5 kb-BamHI DNA fragment and produced three extacellular proteases. Their protease properties corresponded to those of Xanthomonas sp. JK311. |
| Download PDF | Eng_330414_344-349p.pdf |