| Title | Construction of secretion vectors using the α-amylase signal sequence of bacillus subtilis NA64 |
|---|---|
| Author | Kim, Sung Il · Lee, Se Yong * |
| Address | Department of Agricultural Chemistry, College of Natural Resources, Korea University |
| Bibliography | Journal of Microbiology, 34(1),74-81, 1996, |
| DOI | |
| Key Words | B. subtilis, secretion vector, β-lactamase secretion |
| Abstract | Two secretion vectors, pUBA240 and pUB340 were constructed by using the promoter and secretory signal region of the α-amylase gene from an α-amylase hyperproducing strain, Bacillus subtilis NA64. In this secretion vector system, various restriction enzyme sites are located immediately after the proregion of the α-amylase gene for easy replacement of various foreign structural genes. To evaluate this secretion vectors, the β-lactamase gene of pBR322 was used as a reporter gene. The expressed and biologically active β-lactamase was secreted into the culture broth from B. subtilis LKS86 transformants harboring each β-lactamase secreting plasmid, pUBAbla and pUBSble. In both cases, more than 92% of expressed β- lactamases were located idn the culture medium. The amount of the secreted β-lactamase was about 80% of the total secreted proteins in the culture medium. |
| Download PDF | Eng_340112_74-81p.pdf |