| Title | Purification of carbosymethyl cellulase from hybrid between aspergillus niger and penicillium verruculosum |
|---|---|
| Author | Yang, Young Ki * · Lee, Jung Sup · Park, Hyung Nam · Moon, Myung Nim · Kim, Hong Sub¹ · Kim, Jong Se¹ · Lim, Chae Young · Rhee, Young Ha² |
| Address | Department of Genetic Engineering; ¹Department of Biology, College of Natural Science, Chosun University; ²Department of Microbiology, College of Natural Science, Chungnam National University |
| Bibliography | Journal of Microbiology, 34(1),90-94, 1996, |
| DOI | |
| Key Words | Aspergillus niger, Penicillium verruculosum, TAPW157-3, carboxymethyl cellulase, purification |
| Abstract | The carboxymethyl cellulase (CMCase) was purified from the induced culture filtrate of hybrid TAPW15703 between Aspergillus niger and penicillium verruculosum made by nuclear transfer. The enzyme was purified 80 fold with an overall yield 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and DEAE-ion exchange column chromatography. The molecular weight of the CMCase has estimated to be 32,000 daltons on SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel permeation chromatography. The purified enzyme functions optimally at pH 4.0 and 40℃. The Km value for carbosymethyl cellulose was 68 mM. The enzyme activity was increased by the presence of Mg^2+ and Mn^2+. |
| Download PDF | Eng_340114_90-94p.pdf |