Title Detection of L-xylosone and its physiological effects in saccharomyces cerevisiae
Author Seok, Yeong Jae · Yang, Kap Seok · Kang, Ju Gyeong · Kim, Seong Tae · Huh, Won Ki · Kang, Sa Ouk *
Address Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular microbiology, Seoul National University
Bibliography Journal of Microbiology, 34(2),192-197, 1996,
Key Words L-xylosone, α-ketoaldehyde, L-ascorbic acid, dehydro-L-ascorbic acid, cell growth, Sacchromyces cerevisiae
Abstract L-Xylosone was detected as its quinoxaline derivative in the degradation solution of dehydro-L-ascorbic acid. The production rate of L-xylosone was much faster in aerated phosphate-cirate buffer (pH 5. 6) than in pure water. L-Xylosone and dehydro-L-ascorbic acid were identified in the crude extracts of Saccharomyces cerevisiae. The concentration of L-xylosone in the crude cell extracts was calculated to be about 0.2 nmol (mg protein)^-1. When L-xylosone was added to asynchronous culture of S. cerevisiae, it inhibited primarily the synthesis of protein and RNA. Examination of the effect of L- xylosone on synchronous culture of the yeast indicated that L-xylosone inhibited the initiation of DNA replication and that the cells were arrested at G₁stage of cell division cycle. These results suggested a possibility that L-xylosone can act as an inhibitor of cell growth.
Download PDF Eng_340213_192-197p.pdf