| Title | Immunofluorescence localization of schizosacharomjyces pombe cdc103^+ gene product |
|---|---|
| Author | Kim, Hyong Bai |
| Address | Division of Microbialtechnology, Graduate School of Biotechnology, Korea University, Seoul; Department of Biotechnology, Korea University, Chung Nam |
| Bibliography | Journal of Microbiology, 34(3),248-254, 1996, |
| DOI | |
| Key Words | Schizosaccharomyces pombe, Saccharomyces cerevisiae, cdc103^+, CDC3 |
| Abstract | cdc103^+ gene in Schizosaccharomyces pombe which is similar to the CDC3 gene in Saccharomyces cerevisiae was cloned and sequenced. Comparison of the predicted amino acid sequences of cdc103^+ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other. The gene product of CDC3 S. cerevisiae is known to be a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. In order to characterize the gene product of cdc103^+ in Schizosaccharomyces pombe, fusion proteins were used to generate the polyclonal antibodies specific for the gene product (cdc103p). In immunofluorescence experiments, these antibodies decorate the region of the septum formation as a double ring structure late in the cell division cycle. |
| Download PDF | Eng_340307_248-254p.pdf |