| Title | Lipoppolysaccharide yields form Rhodobacter capasulatus with indirect ELISA |
|---|---|
| Author | Yoo, Tae Eun · Lee, Hyun Soon * |
| Address | Department of Biology, Sung Kyun Kwan University |
| Bibliography | Journal of Microbiology, 34(3),255-262, 1996, |
| DOI | |
| Key Words | Rhodobacter capsulatus, LPS, ELISA method, affinity chromatography |
| Abstract | The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to NH₄CI concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM CaCI₂, the LPS yield was 16.5 ㎍/mg DW, five times the yield without calcium. |
| Download PDF | Eng_340308_255-262p.pdf |