| Title | Calcium in infectious hematopoietic necrosis virus (IHNY) infected fish cell lines |
|---|---|
| Author | Kim, Nam Shik · Heo, Gnag Joon¹ · Lee, Chang Hee * |
| Address | Department of Microbiology, and ¹Department of Veterinary Medicine, Chungbuk National University |
| Bibliography | Journal of Microbiology, 34(3),263-269, 1996, |
| DOI | |
| Key Words | Infectious Hematopoietic Necrosis Virus(IHNV), calcium, fish cell lines |
| Abstract | Infection of fish cells with IHNV resulted in gradual increase in cytosolic free Ca^2+ concentration ([Ca^2+]) in CHSE, gradual decrease in [Ca^2+] in FHM, and no significant change in RTG cells. The degree of [Ca^2+] increase or decrease was dependent on the amount of infectious virus, and these [Ca^2+] variations were maximal at 16 hours after virus infection (p.i.) in both cell lines. When the fish cells were infected with inactivated IHNV, evident variation in [Ca^2+] was not observed. Thus, infectivity of IHNV appears to correlate with changes in [Ca^2+] in virus-infected cells. These IHNV-induced [Ca^2+] changes were partially blocked by cycloheximide, but not affected by cordycepin. It seems to be that virus-induced Ca^2+ variations were more related with protein synthesis than RNA synthesis. Various Ca^2+ related drugs were used in search for the mechanisms of the [Ca^2+], changes following IHNV infection of CHSE cells. Decreasing extracellular Ca^2+ concentration or blocking Ca^2+ influx from extracellular media inhibited the IHNV-induced increase in [Ca^2+], in CHSE cells. Similar results were obtained with intracellular Ca^2+ sources are important in IHNV-induced [Ca^2+] increase in CHSE cells. |
| Download PDF | Eng_340309_263-269p.pdf |