| Title | Fungal-sporulation suppressing substances produced by pseudomonas aeruginosa KMCS-1 |
|---|---|
| Author | Min, Bu Yong · Shim, Jae Young · Kim, Kun Woo² · Lee, Jong Kyu¹ · Yoon, Kwon Sang * |
| Address | Department of Microbiology, ¹Department of Forest Resource protection, kangwon National University; ²Department of Resources Plant, Andong National University |
| Bibliography | Journal of Microbiology, 34(3),284-288, 1996, |
| DOI | |
| Key Words | Pseudomonas aeruginosam antifungal substance, asexual sporulation, silica fel chromatography, bioassay |
| Abstract | Among the bacteria isolated form compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae; however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 ㎍/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted. |
| Download PDF | Eng_340313_284-288p.pdf |