| Title | Isolation, characterization, and phylogenetic position of a new sulfur-oxidizing bacterium |
|---|---|
| Author | Chang, So Youn · Yoon, Joon Sik¹'⁴· Shin, Yong Kook⁴· Park, Yong Ha⁴· Park, Jin Yeol · Yang, Song Suk · Koh, Moon Joo² · Yoon, Seong Myeong³ · Lee, Jung Sup¹ · Lee, In Hwa · Kim, Si Wouk * |
| Address | Department of Environmental Engineering; ¹Department of Biological Science; ²Department of Chemistry; ³Department of Biology Education, Chosun University; ⁴Korean Collection for Type cultures, Korea Research Institute of Bioscience and Biotechnology |
| Bibliography | Journal of Microbiology, 35(3),165-171, 1997, |
| DOI | |
| Key Words | Facultative chemolithoautotroph, sulfur-oxidizing bacterium, Thiobacillus |
| Abstract | A sulfer-oxidizing bacterium was isolated from mine wastewater and characterized. The isolate was gram-negative, rod (0.2 × 1.2-1.5㎛), nonmotiloe, catalase positive, and oxidase positive. The optimal pH and temperature for growth were 7.0 and 30℃, respectively. The optimum thiosulfate concentration was 70 mM and the maximum growth rate was 0.081 hr. The major ubiquinone contained in the isolate was Q-8. The cellular fatty acid composition was C_16:0, C_18:1, C_17cyc, and C_19cyc as nonpolar fatty acids, and 3-OH C10 : 0 and 3-OH C_12:0 as hydroxylated fatty acids. The isolate was a facultative chemolithoautotroph which can grow autotrophically on sodium thiosulfate and sodium sulfide and which can grow heterotrophically on yeast extract. It can also grow mixotrophically on sodium thiosulfate and yeast extract. Comparison of the 16S rRNA gene sequence of the isolate with that of Thiobacillus species and Paracoccus thiocyanatus revealed that it is closely related to T. caldus which belongs to the β-subclass of the class Proteobacteria. However, the isolated could not grow at extremely low pH (pH 1-3.5). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolate was tentatively named Thiobacillus sp. strain C. |
| Download PDF | Eng_350302_165-171p.pdf |