| Title | Isolation of pseudomonas sp. S-47 and its degradation of 4-chlorobenzoic acid |
|---|---|
| Author | Seo, Dong In · Lim, Jai Yun · Kim, Young Chang · Min, Kyung Hee¹ · Kim, Chi Kyung * |
| Address | Department of Microbiology, Chungbuk National University; ¹Department of Biology, Sookmyung Women's University |
| Bibliography | Journal of Microbiology, 35(3),188-192, 1997, |
| DOI | |
| Key Words | 4-chlorobenzoic acid, degradation, Pseudomonas sp. S-47 |
| Abstract | The strain of S-47 degrading 4-chlorobenzoic acid (4CBA) was isolated from Ulsan chemical industrial complex by enrichment cultivation with 1 mM 4CBA. The strain was Gram-negative rod and grew optimally at 30℃ and pH 7 under aerobic condition, so that the organism was identified as a species of Pseudomonas. Pseudomonas sp. S-47 degraded 4-chlorobenzoic acid to produce a yellow-colored meta-cleavage product, which was confirmed to be 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by UV-visible spectrophotometry. 5C-3HMS was proved trometry. This means that Pseudomonas sp. S-47 degraded 4CBA via 4-chlorocatechol to 5C-2HMS by meta-cleavage reaction and then to 5C-2HMA by 5C-2HMS dehydrogenase. |
| Download PDF | Eng_350306_188-192p.pdf |