| Title | Cloning and phylogenetic analysis of chitin synthase gene from entomopathogenic fungus, beauveria brongniartii |
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| Author | Nam, Jin Sik · Lee, Dong Hun · Park, Ho Yong · Bae, Kyung Sook * |
| Address | Genetic Resources Center, Korea Research Institute oif Bioscience and Biotechnology |
| Bibliography | Journal of Microbiology, 35(3),222-227, 1997, |
| DOI | |
| Key Words | Breauveria brongniartii, chitin synthase gene, PCR, phylogeny |
| Abstract | DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHS1. Comparison of the deduced amino acid sequence of BbCHS1 with those of other Euacomycetes revealed that quence of BbCHS1 displayed the highest rate of similarity, 95.8%, with CHS2 or Metarhizium anisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongiartii, which was previously derived by traditional fungel clasification based on morphological features. |
| Download PDF | Eng_350311_222-227p.pdf |