| Title | A conditional lethal mutation of a nucleoporin gene, NUP49 in saccharomyces cerevisiae |
|---|---|
| Author | Lee, Youn Soo · Song, Young Ja · Hwang, Mi Kyung · Lee, Woo Bok · Kim, Jin Mi * |
| Address | Department of Microbiology, College of Natural Sciences, Chungnam National University |
| Bibliography | Journal of Microbiology, 35(3),234-238, 1997, |
| DOI | |
| Key Words | Nucleoporin gene, yeast, temperature-sensitivity, nuclear pore |
| Abstract | Conditional lethal mutation nup49-1 of a nuclear pore complex component gene was constructed in Saccharomyces cerevisiae. This mutation deleted one third of the essential NUP49 gene at the carboxy-terminal, but retained 13 repeats of the highly conserved GLFG domain. The nup49-1 mutant strain was viable with a slow-growth phenotype, indicating that the C-terminal is dispensable at normal growth temperature. This strain exhibited both temperature-sensitivity at 37℃ and cold-sensitivity at 16℃. Temperature shift experiments revealed that the arrest phenotype at 37℃ was random in the cell division cycle. The nup49-1 mutation was tested to be recessive and is expected to be useful for the functional analysis of nuclear pore complex proteins as well as for studies of nuclear transport systems. |
| Download PDF | Eng_350313_234-238p.pdf |