Title |
rpoS mutation relieves biosynthesis of flagella in hns mutants of salmonella typhimurium UK1 |
Author |
Cho, Mi Ook · Bang, Ile Soo · Hong, Seong Karp¹ · Bang, Seong Ho¹ · Park, Yong Keun * |
Address |
Graduate School of Biotechnology, Korea University; ¹Department of Biology, Hanseo University |
Bibliography |
Journal of Microbiology, 36(3),184-188, 1998,
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DOI |
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Key Words |
Flagella, hns, rpoS/hns, Salmonella typhimurium |
Abstract |
The biosynthesis and function of flagella is positively regulated by the cyclic AMP-catabolite activator protein (cAMP-CAP) complex and the nucleoid protein H-NS. In this report, we show that nonmotile Salmonella typhimurium hns mutants could recover its motility by introducing the rpoSmutation. In a swarm plate assay, rpoS/hns double mutants could woim while hns mutants could not. This regeneration of motility resulted from the flagella synthesis. Transmission electron microscopy analysis showed the capability of rpoS/hns double mutants for flagella synthesis. And rpoS mutation derepressed the transcription of flhD, the flagella master gene, in hns mutants. |
Download PDF |
Eng_360306_184-188p.pdf |